Fluorescein - Fluorescein album download
It is widely used as a fluorescent tracer for many applications. Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm and emission maximum of 512 nm (in water)
Fluorescein is a fun dye: the solid crystals are red while its dilute solutions are yellow-green. This is due to the red (stable) form containing a carboxylic acid group, which promptly deprotonates in solution to form an intramolecular twisted cyclic ester (unstable), that is yellowish green. ww. heskepticalchemist.
Fluorescein Lyrics Abandoned Pools. Abandoned Pools - Fluorescein Songtext
The fluorescence emission spectrum of Fluorescein dissolved in basic ethanol. The excitation wavelength was 470nm. The quantum yield of this molecule is . 7 (Seybold, 1969b). This spectrum was collected by on 06-20-1995 using a Spex FluoroMax
There are many fluorescein derivatives, for example fluorescein isothiocyanate, often abbreviated as FITC. In the FITC molecule, an isothiocyanate group (-N C S) replaces a hydrogen atom on the bottom ring of the fluorescein molecule. This derivative is reactive towards amine groups on proteins inside cells. Fluorescein-labeled probes can be imaged using a technique known as FISH (fluorescent in situ hybridization), or targeted by antibodies using. The latter is a common alternative to digoxigenin, and the two are used together for labeling two genes in one sample. Ophthalmic applications.